Is it possible to modify QIAsymphony protocols? Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol? Bar code reading of samples and reagents enables full tracking throughout the entire purification and assay setup procedure. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using QIAsymphony DNA Kits. QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. During the QIAamp DNA purification procedure, DNA binds specifically to the QIAamp MinElute or QIAamp silica-gel membrane while contaminants pass through. For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml) You will also require the tool to change the O-rings. It is particularly suitable for short digestion times. What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit? If you have only a QIAsymphony SP instrument, only the QIAsymphony Cabinet SP is required. Find the right products for every step of your experiment effortlessly. Such fluorescence is typically attributable to the use of a degraded, dual-labeled probe. Therefore an additional incubation step in buffer ATL containing Proteinase K is included in the Tissue Protocol to achieve complete sample lysis. QIAsymphony Kits; EZ1 Kits; Whole Genome Sequencing; Whole Exome Sequencing; Targeted DNA Panels; Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Cell-Free DNA; DNA Clean Up; Genomic DNA; This joint effort between NCI and the National Human Genome Research Institute began in 2006, bringing together researchers from diverse disciplines and multiple institutions. We have no plans to introduce QR code recognition any time soon. Buffers and reagents for use with QIAGEN products. Yes. As an integrated system in combination with the QIAsymphony AS, the QIAsymphony SP can automate workflows from sample to PCR setup ready for amplification. The QIAamp DNA Blood Mini Kit procedure is This site is protected by reCAPTCHA and the Google, Efficient purification of DNA from small sample sizes. It helps to reduce possible pathogen contamination of the QIAsymphony SP/AS worktables. For automated purification of intracellular RNA (including miRNAs) from stabilized blood. QIAsymphony Bisulfite Kit: For molecular biology applications: 931106: For sample lysis and complete bisulfite conversion/cleanup of DNA using the QIAsymphony SP, optimized for downstream methylation analysis: DNA: QIAsymphony DNA Investigator Kit: For molecular biology applications: 931436: Soluble calcium is not essential for enzymatic activity. How can I determine if a tube is suitable for clot detection on QIAsymphony? Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.Pleaseaccessour Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit. Using the Standard protocol for RNA extraction on the QIAsymphony SP, up to 400 l of lysate can be processed while theLarge-Volume protocol allows up to800 l of homogenate. Buffer ATL. The maximum amount of tissue that can be processed on the QIAsymphony SP using QIAsymphony DNA Mini Kit is 50 mg, and the maximum number of cultured cells is 1 x 107. Do you have a protocol for the isolation of genomic DNA from bone? The RLT buffer is more viscous than the RLT plus buffer. For tissue lysis. Investigator Lyse&Spin Basket Kitscolumns can be used to increase recovery from some sample types. For automated purification of intracellular RNA (including miRNAs) from stabilized blood. After pretreatment, 220 l of the lysates must be transferred to the. Buffer AL is required in both protocols to ensure appropriate conditions for DNA binding to the silica membrane. Is there a charge for user training on the QIAsymphony SP System? Streamlined protocols with optimized RNA extraction reagents simplify handling and are optimized for your specific sample types, formats and throughputs, as well as for manual and automated processing. The QIAsymphony AS instrument is designed to perform automated assay setup in molecular diagnostic and/or molecular biology applications. QIAcube HT and QIAxtractor. For automated purification of DNA from 196 samples. Find protocols, product documentation, software, FAQs, videos, webinars, and more. Response time of next business day. 10 mM Tris-Cl pH 8.5 to 9.0) to prevent DNA degradation by acid hydrolysis. Extending the QIAsymphony SP with the QIAsymphony AS results in a fully integrated system that can automate sample preparation and assay setup workflows without manual interference. RNeasy Lipid Tissue Mini Kit. In combination with QIAsymphony DSP Kits (shown above) it automates nucleic acid purification in research and IVD (in vitro diagnostic) workflows. When purifying RNA, it is critical to use an optimized method for your sample type. Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol? Select your lab focus area to learn more about our high-quality products and comprehensive workflows for your specific applications. For purification of cell-free DNA and RNA from plasma and serum samples. no 990394, and the required disposable tips: Filter-Tips, 200 l (1024), cat no. A large-volume protocol is available that enables processing of larger sample amounts. A no template control (NTC) omits any DNA or RNA template from a reaction, and serves as a general control for extraneous nucleic acid contamination. QIAcube HT and QIAxtractor. QIAamp DNA FFPE Tissue Kit. Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? high-precision PCR setup, while the QIAsymphony AS is ideal for laboratories performing routine PCR tests on a day-to-day basis. The specified lysate volumes are optimal for QIAsymphony DNA Investigator Kit protocols. Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit? In addition to the purification kit, Rotor Adapters (10 x 24), cat. Buffer ATE of theQIAsymphony DNA Investigator Kit is a low-EDTA elution buffer optimized for long-term storage of DNA as well as performance in sensitive downstream applications. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. In general, magnetic particles are not carried over into QIAsymphony eluates. Customized protocols for QIAsymphony SP will be available on request. QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. QIAsymphony Kits; EZ1 Kits; Whole Genome Sequencing; Whole Exome Sequencing; Targeted DNA Panels; Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Cell-Free DNA; DNA Clean Up; Genomic DNA; For extraction of total cellular DNA from plant cells and tissues or fungi, or genomic DNA from plant cells, tissues and seeds. no. The TRACKMAN Connected system guides researchers through the QIAamp DNA Mini protocols while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Do you need improved connectivity? Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle? Elution in smaller volumes will provide higher concentration, but lower overall yield. If isolation of RNA-free DNA is required, RNase A must be added to the lysate before isolation of DNA. The sample input drawer accommodates a wide range of sample input formats to suit varying workflows and sample preparation requirements. No. QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite or viral DNA from human tissue samples ready to use in PCR and blotting procedures. The convenient spin-column procedure means that hands-on preparation time is only 20 minutes (lysis times differ according to the sample source). Can uneven sample numbers be processed on the QIAsymphony SP module? 303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit? Find protocols, product documentation, software, FAQs, videos, webinars, and more. A handbook for high-level expression and purification of 6xHis-tagged proteins The 3 most common negative controls included in a qPCR and/or qRT-PCR experiment are as follows: 1. What RNA elution volumes are available with the QIAsymphony RNA Kit? The concentration of carrier DNA should be at least 10 g/ml. The ACS specifies the combination of a protocol and a predefined carrier RNAinternal control mixture. No, currently only barcodes can be used to label samples for QIAsymphony. Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction? The barcode on the adapter at the cooling elution position is read by default. Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Histone Modification; QIAsymphony Kits; EZ1 Kits; EZ2 Kits; Tissue Disruption; Informatics & Data. For purification of RNA from blood on the QIAsymphony SP, we recommend using the QIAsymphony PAXgene RNA Kit, since it includes stabilization of gene expression profiles. Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Histone Modification; QIAsymphony Kits; EZ1 Kits; EZ2 Kits; Tissue Disruption; Informatics & Data. For more information, refer to the QIAsymphony User Manual. What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits? If magnetic particles need to be removed before performing downstream applications, tubes or plates containing eluates should first be placed in a suitable magnet and the eluates transferred to a clean tube. Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)? --|MinElute PCR Purification KitbaPCR1.2% TAEM|GelPilot Loading Dye3DNA| Simply load drawers with the required samples, reagents, and consumables. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Can I still use them? DNA of this length denatures completely and has the highest amplification efficiency. On-site QIAsymphony AS instrument repair, including travel, labor and parts, for a period of one year. 1 x 50 ml Deparaffinization Solution for use with QIAsymphony DSP DNA Mini Kit FFPE tissue protocols. QIAcube HT and QIAxtractor. The kit includes rotor adapters that are preloaded with QIAamp spin columns and elution tubes, delivering greater convenience and time savings (see figure "Significant time savings"). 990332, Filter-Tips, 1000 l (1024), cat no. Pure RNA should yield an A260/A230 ratio of around 2 or slightly above; however, there is no consensus on the acceptable lower limit of this ratio. RNeasy Plant Mini KitRNA10100 mg1001 x 10 7 "RNeasy Plant Mini Kit" 100 gRNA Sample handling, software, and introduction to maintenancefor the QIAsymphony SP Systemare included in the installation training and are covered by the installation fee. QIAsymphony Bisulfite Kit: For molecular biology applications: 931106: For sample lysis and complete bisulfite conversion/cleanup of DNA using the QIAsymphony SP, optimized for downstream methylation analysis: DNA: QIAsymphony DNA Investigator Kit: For molecular biology applications: 931436: A convenient tool to build experimental workflows and find products to match your needs. The QIAamp DNA Mini Kit is ideal for purification of DNA from most commonly used human tissue samples, including muscle, liver, heart, brain, bone marrow and other tissues, swabs (buccal, eye, nasal, pharyngeal and others), CSF, blood, body fluids and washed cells from urine. How should DNA isolated on the QIAsymphony be stored? Samples must be processed manually. The size of DNA obtained with QIAamp DNA Mini and QIAamp Blood Mini kit ranges between 100 bp and 50 kb, with 30 kb fragments predominating. Power consumption of a QIAsymphony SP instrument is 800 VA. The efficiency of downstream applications depends strongly on the purity of the RNA sample used. QIAfilter Plasmid Maxi Kit. This tool setincludes onebag of 32 O-rings. The QIAsymphony SP is ready-to-run and requires only minimal installation. This removes co-precipitated salt and replaces the isopropanol with the more volatile ethanol, making the DNA easier to redissolve. What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR ").Purified DNA may also be used in short-tandem repeat 1048146. Worktable setup is rapid and saves you valuable time. 990352, Response time of two business days. QIAamp ccfDNA/RNA Kit. Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Histone Modification; QIAsymphony Kits; EZ1 Kits; EZ2 Kits; Tissue Disruption; Informatics & Data. How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP? However, for optimal performance and quality, storage temperature should not exceed 25C. QIAamp ccfDNA/RNA Kit. No. At a later date, users will be able to import sample worklists to the QIAsymphony SP. Blood cells will directly lyse by incubation in Buffer AL containing QIAGEN Protease. DNeasy Plant Mini Kit DNADNA DNeasy Plant Kit Carrier RNA is not used in reference protocols. Extraction will work fine, but there may be some magnetic bead carryover to the eluate. Yes, clot detection is possible on the QIAsymphony. Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP? No mechanical homogenization is necessary as the tissues are lysed enzymatically. Yes, please follow the User-Developed Protocol 'Purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit' (QA43). The QIAamp DNA Mini Kit provides silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids, or washed cells from urine. No. DNA was purified from blood samples (164 l) using the QIAamp DNA Micro Kit following the blood protocol. The QIAsymphony SP instrument is designed to perform automated purification of nucleic acids in molecular diagnostic and/or molecular biology applications. The QIAamp DNA Mini Kit provides silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids, or washed cells from urine. Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit? For purification of miRNA and total RNA from tissues and cells, For purification of total RNA from plants and fungi, For purification of cellular RNA from fresh whole blood, For purification of total RNA from formalin-fixed, paraffin-embedded tissue sections, For automated high-throughput isolation of RNA from animal and human cells and tissue, For efficient lysis of fatty and standard tissues before RNA isolation, For RNA cleanup and concentration with small elution volumes, For rapid, easy, high-throughput mRNA purification from cells, For automated purification of total RNA, or total RNA with miRNA, from tissues or cells, For automated purification of total RNA, including miRNA, from serum and plasma samples, For phenol-free isolation of total RNA including miRNA from serum and plasma samples, For purification of cell-free total RNA, including miRNA, from animal and human plasma and serum, For purification of up to 100 g total RNA from fatty tissues and other types of tissue, For purification of miRNA and total RNA from whole blood, For isolation and purification of intracellular RNA from blood stabilized in PAXgene Blood RNA Tubes, For purification of total RNA from all types of tissue, For immediate stabilization of RNA in tissues, cultured cells and bacterial cultures, For purification of up to 100 g total RNA from fiber-rich tissues, For the automated, hands-free isolation of nucleic acids from stool and gut material, For efficient isolation of exosomal RNA from biofluids, For the isolation of high quality total RNA from all soil types, For purification of microRNA and total RNA from formalin-fixed, paraffin-embedded tissue sections, For the isolation of total RNA from stool and gut material, For high-throughput purification of cellular RNA from whole blood, For stabilization and purification of total RNA from tissues, cells, human saliva and bacteria, For automated purification of intracellular RNA (including miRNAs)from stabilized blood, For purification of cell-free DNA and RNA from plasma and serum samples, For automated purification of high-quality total RNA, For purification of total RNA from formalin-fixed paraffin embedded (FFPE) tissue sections for in vitro diagnostic use, For 96-well purification of total RNA from cells or any tissue on the BioRobot Universal System, For isolating total RNA from plant and seed samples, including those high in polyphenols and polysaccharides. All solid materials (e.g., swabs, blood card punches, chewing gum) must be removed completely from the lysate before performing automated extraction on the QIAsymphony SP. Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Histone Modification; QIAsymphony Kits; EZ1 Kits; EZ2 Kits; Tissue Disruption; Informatics & Data. Select your lab focus area to learn more about our high-quality products and comprehensive workflows for your specific applications. On-site QIAsymphony SP instrument repair, including travel, labor and parts, for a period of one year. Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of theQIAGEN Genomic DNA Handbook. When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit? Therefore, the approximate thermal output is 2729 BTU/h. Magnetic particles used in the QIAsymphony SP System will bind RNA and total DNA. The QIAsymphony SP and the QIAsymphony SP/AS instruments deliver state-of-the-art process safety and standardization features. Fragments of approximately 200 bp can be isolated with good recovery. The QIAsymphony AS provides active cooling of reagents, eluates and assays, enabling safe and reproducible assay setup. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 l, with a preparation time of 2040 minutes. When purifying small amounts of DNA using. How do I perform a DNA precipitation to concentrate my sample? When using internal controls, they are added to the carrier RNA mixture (total volume of the mixture must be 120 l). Please contact Technical Service if you wish to use sample tubes not listed in the labware document. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples. DNA yields strongly depend on the type and source of the tissue processed on the. DNA was eluted in 20 l Buffer AE and 2 l of each eluate was amplified by real-time PCR. For automated purification of high-quality total RNA. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. What is the range of elution volumes on the QIAsymphony SP? Explore our digital magazine full of customer stories and videos on scientific breakthroughs and healthcare advances. The QIAlink software which is available separately (?) Does the QIAsymphony SP system generate run reports and/or log files? Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Histone Modification; QIAsymphony Kits; EZ1 Kits; EZ2 Kits; Tissue Disruption; Informatics & Data. What is the thermal output for a QIAsymphony SP and AS instrument? How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony? DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR ").Purified DNA may also be used in short-tandem repeat These products are not intended for the diagnosis, prevention, or treatment of a disease. What are the dimensions and weight of the QIAsymphony SP System? QIAEX II Gel Extraction KitDNA40 bp50 kb6095% 10 l QIAEX II5 gDNA20 l QIAamp DNA Mini KitDNAPCRPCRSNPSTR 24/7 automatic processing of online orders, Knowledgeable and professional Product & Technical Support. Buffer ATE contains a small amount of the preservative sodium azide. QIAcuity. For most sample types, incubating longer than specified in the pretreatment protocol for the QIAsymphony DNA Investigator Kitdoes not increase yields. The QIAsymphony AS enables setup of single or multiple PCRs per run or sample, splitting of eluates for panel testing, and active cooling of eluates, reagents, and PCRs. Alternatively, casework protocols using water for elution are available. The EpiTect HRM PCR Kit enables fast screening and accurate detection of changes in CpG methylation status of bisulfite converted DNA. Yields of nucleic acids or DNA depend on the starting material (see table Typical yields with the QIAamp DNA Mini Kit). 1 x 50 ml Deparaffinization Solution for use with QIAsymphony DSP DNA Mini Kit FFPE tissue protocols. The dedicated range of optimized QIAsymphony kits and protocols guarantees best purification efficiency and nucleic acid quality and increases laboratory capabilities/flexibility with a single instrument. EDTA helps to prevent any nuclease activity introduced after the genomic DNA extraction procedures. For purification of viral RNA from plasma, serum, cell-free body fluids, cell-culture supernatants Multiplex amplification for human ID, with CODIS and European standard markers. When using SYBR Green chemistry, this also serves as an important control for primer dimer formation. Download more information. Lysis time varies depending on the tissue type processed. Ready-to-run, bar code-labeled reagent cartridges are prefilled with all reagents required for the purification procedure, including accessory enzymes (see figure "Prefilled, sealed reagent cartridges"). QIAsymphony DNA Investigator Kit. A convenient tool to build experimental workflows and find products to match your needs. Investigator Lyse&Spin Basket Kitscolumns can be used to increase sample recovery from some solid materials. The typical yield from 200 l healthy whole blood is 412 g, with an elution volume of 50200 l. For purification of cell-free DNA and RNA from plasma and serum samples. For use as a resuspension buffer when preparing plasmid DNA. The target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony is viral DNA from released as well as cell-associated viruses, co-purified with genomic DNA from blood cells. DNA, RNA, protein, whole genome amplification, next-generation sequencing. The 3 most common negative controls included in a qPCR and/or qRT-PCR experiment are as follows: 1. Response time of two business days. PAXgene Blood miRNA Kit. Any combination of standard and large-volume sample preparation can be performed. Dedicated QIAcube kits are the RNeasy Mini QIAcube kit, QIAamp DNA Mini QIAcube Kit, the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit. Which QIAGEN DNA extraction kits are compatible with the PAXgene Saliva Collector? The MinElute spin columns included in the following kits should be stored at 28C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro. Enjoy over 30 free expert talks covering seven major life science areas. Bisulfite Conversion Assays; Methylation-Specific PCR; Pyrosequencing; Histone Modification; QIAsymphony Kits; EZ1 Kits; EZ2 Kits; Tissue Disruption; Informatics & Data. The QIAsymphony SP/AS instruments provide standardized communication interfaces. Up to 90 g DNA can be purified from tissues with high cell densities (e.g., spleen) or large numbers of cultured cells. The initial centrifugations are performed at 6000 x g simply to reduce centrifuge noise. Frozen samples must be thawed thoroughly before use. For purification of viral RNA from plasma, serum, cell-free body fluids, cell-culture supernatants Please contact Technical Service for this protocol. This will not negatively affect the QIAamp Procedure, sincethe enzyme will be efficiently removedby the wash steps in the protocols. The expected yield from 1 ul of whole blood with 4000-7000 leukocytes is 30 ng of genomic DNA. The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1100 l whole blood, blood cards of ~3 mm diameter, urine or <10 mg tissue or laser microdissected tissue). For purification of RNA on the QIAsymphony SP,tissue samples require mechanical disruption and homogenization in the supplied lysis buffer (Buffer RLT Plus), e.g., by using the TissueLyser or TissueRuptor. For more information, see the corresponding pretreatment protocol. All steps in the purification procedure are fully automated up to 12 samples can be processed per run. Plasma, serum, and CSF samples can be processed using the Virus Cellfree protocols of the QIAsymphony Virus/Bacteria Kits. Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? RNeasy Plant Mini KitRNA10100 mg1001 x 10 7 "RNeasy Plant Mini Kit" 100 gRNA (EN) - QIAamp DNA Accessory Sets June 2012, Product Sheet QIAamp DNA Mini QIAcube Kit, QIAGEN-Gilson Digitalized Pipetting and Protocols presentation, Product Profile - QIAamp genomic DNA kits, QIAGEN-Gilson Digitalized Pipetting and Protocols flyer, Quick-Start Protocol: QIAamp DNA Mini Kit, Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 2 - (EN), Purification of genomic DNA from bones using the QIAamp DNA Micro Kit - (EN), Purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit - (EN), Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 1 - (EN), Isolation of genomic DNA from fungi (culture and blood) using the QIAamp DNA Mini Kit - (EN), Isolation of DNA from soft tissues using the TissueLyser and QIAamp DNA Mini Kit - (EN), Acetyl Cysteine (NALC) treatment of viscous samples - (EN), Isolation of genomic DNA from compact bone using the QIAamp DNA Mini Kit - (EN), Isolation of genomic DNA from nails and hair using the QIAamp DNA Mini Kit - (EN), Handling instructions for multiple sample preparations using the QIAamp DNA Mini Kit - (EN), Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp DNA Micro Kit - (EN), Purification of REPLI-g amplified DNA using the QIAamp DNA Mini Kit - (EN), Simultaneous Purification of DNA and RNA from Saliva Stabilized in RNAprotect Saliva Reagent - (EN), Purification of DNA from Saliva Stabilized in RNAprotect Saliva Reagent - (EN), QIAcube Protocol Sheet: PAXgene Saliva spin protocol V1 for extraction of gDNA from 200 l PAXgene Saliva Collector samples, High-quality genomic DNA isolation and sensitive mutation analysis, Acetyl Cysteine (NALC) treatment of viscous samples, Acetyl Cysteine (NALC) treatment of viscous samples using the DNeasy Blood & Tissue Kit, 'Isolation of DNA from soft tissues using the TissueLyserand QIAamp DNA Mini Kit, Purification of total DNA from soft tissues using the TissueLyser and the DNeasy Blood & Tissue Kit, QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook, http://www.qiagen.com/Products/Catalog/Automated-Solutions/Sample-Prep/QIAcube#orderinginformation, Purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit, QIAamp DNA Mini and QIAamp DNA Blood Mini Kit Handbook, Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp DNA Micro Kit, Purification of REPLI-g amplified DNA using the QIAamp DNA Mini Kit, Nucleic acid yield without RNase A treatment (ug), No organic extraction or alcohol precipitation, Complete removal of contaminants and inhibitors, Automated purification of 112 samples on the QIAcube Connect, Isolation of genomic DNA from compact bone using the QIAamp DNA Mini Kit (, Isolation of genomic DNA from compact bone using the DNeasy Blood & Tissue Kit (, Purification of genomic DNA from bones using the QIAamp DNA Micro Kit (, Purification of archive-quality DNA from bone fragments using the Gentra Puregene Tissue Kit (, Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; vacuum procedure (, Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; spin procedure (, Isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit; spin procedure (.

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